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Antibiofilm Activity of Fructophilic Lactic Acid Bacteria filtrate against Klebsiella Pneumoniae Virulence Gene Expression

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Last updated: 03 Jan 2025

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Abstract

Background: Fructophilic lactic acid bacteria (FLAB) are a group of LAB with unique growth features and are regarded as potential bioactive compound manufacturers. FLAB is discovered in D- fructose rich niches that favors D-fructose as a growth substrate triumphs over D-glucose. On D-glucose, they require electron acceptors to proliferate. The organisms have similar metabolic processes for carbohydrates. Fructobacillus spp < /em>. are Lactobacillus kunkeei, are members of this unique group. Methodology: Inhibitory effect of filtrate from four FLAB isolates from honeybee was detected against the growth of pathogenic Klebsiella pneumoniae isolates. Biofilm formation, and the expression of fimH, mrkD, and NDM-1, and OXA-1 virulence genes were also tested. Results: It was found that FLAB has antibacterial and antibiofilm effect. The incubation of K. pneumoniae with FLAB suspension at different three-time intervals (3, 6, and 9 hrs.) decreased expression of fimH, mrkD, NDM-1, and OXA-1virulence genes compared with the expression of the same genes in the absence of FLAB. Conclusion: all FLAB filtrate isolates tested, K. pneumoniae fimH, mrkD, NDM-1, and OXA-1virulence gene expression was down-regulated, when compared to the control culture.  

DOI

10.21608/ejhm.2022.270278

Keywords

Fructophilic lactic acid bacteria, Klebsiella pneumoniae, Virulence genes, Gene expression, Antibacterial activity

Authors

First Name

Hussam Mahmood

Last Name

Hasan

MiddleName

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Affiliation

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Email

hussam.alammar@sc.uobaghdad.edu.iq

City

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Orcid

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First Name

Ghada Mohammed

Last Name

Salih

MiddleName

-

Affiliation

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Email

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City

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Orcid

-

First Name

Hameed M.

Last Name

Jasim

MiddleName

-

Affiliation

-

Email

-

City

-

Orcid

-

Volume

89

Article Issue

2

Related Issue

37472

Issue Date

2022-10-01

Receive Date

2022-11-17

Publish Date

2022-10-01

Page Start

6,438

Page End

6,444

Print ISSN

1687-2002

Online ISSN

2090-7125

Article File

EJHM_Volume 89_Issue 2_Pages 6438-6444.pdf

PDF . 824.6kB

Link

https://ejhm.journals.ekb.eg/article_270278.html

Detail API

https://ejhm.journals.ekb.eg/service?article_code=270278

Order

58

Type

Original Article

Type Code

606

Publication Type

Journal

Publication Title

The Egyptian Journal of Hospital Medicine

Publication Link

https://ejhm.journals.ekb.eg/

MainTitle

Antibiofilm Activity of Fructophilic Lactic Acid Bacteria filtrate against Klebsiella Pneumoniae Virulence Gene Expression

Details

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Article

Created At

22 Jan 2023