Background: High levels of blood glucose brought on by insulin resistance or deficiency characterize diabetes mellitus type 2, a metabolic condition. One of the main factors contributing to diabetes is viral infection. One of the viruses connected to T2DM is the cytomegalovirus (CMV). Analysis of viral infection and associated factors in a type 2 diabetes sample. Objective: The aim of this study is to measure levels of anti-CMV virus IgG in serum of Iraqi patients with Type 2 diabetes mellitus diseaseand measure the levels of some markers including age, gender, FBS, HbA1c, s. creatinine, blood urea and lipid profile (cholesterol, triglyceride, HLD, LDL, and VLDL). Materials and Methods: This study involved 100 T2DM patients (52 males and 48 females) who visited Al-Karamah Teaching Hospital and Baghdad Teaching Hospital, and 52 healthy Iraqi subjects (control) (26 males and 26 females). In addition to the measures used in this investigation, which also included the lipid profile, HbA1c, FBS, serum creatinine, and blood urea, Anti-CMV IgG was also measured for 48 patients and 40 controls. Results: In comparison with the control group, B. urea, S. creatinine, Anti-CMV IgG, and lipid profile (excluding HDL) were considerably higher in the patient group. While there was no statistically significant difference between groups in terms of age and HDL levels, there was a difference. Conclusion: As indicators and cofactors for type 2 diabetes mellitus, particular laboratory tests and Anti-CMV viral IgG might be utilized.