Background: Bronchial asthma, a common allergic disorder among children, is a chronic airway inflammatory disease. Clinically, it tends to occur with recurrent symptoms mainly involve dyspnea, wheezing, cough, and chest distress. Objective: The aim of the study wasto evaluate the association between lymphotoxin alpha (LTα) gene polymorphisms and childhood bronchial asthma. Patients and methods: A case control study was carried out on 116 children divided to 29 control group and 87 cases. Detection of lymphpotoxin alpha gene polymorphism was assessed. Result: There was statistically significant difference between asthma group and control group regarding genotypes (P-value 0.044). There was a statistically significant negative correlation between FEV1 and IgE level, and a significant positive correlation between FEV1 and eosinophilic count. Conclusion: The genetic predisposition is considered one of the important attributable risk factors of childhood asthma. In addition, the polymorphism of lymphotoxin alpha A/G (rs2844484) is correlated with the risk of childhood asthma.