Background: Intestinal E. vermicularis worm infection is asymptomatic and endemic in Iraq, but it can cause appendicitis; symptoms vary depending on the stage of the gastrointestinal disease. The E. vermicularis worm has caused several cases of appendicitis.  Objective: This study aimed to find out how common E. vermicularis was in appendectomy samples and whether it played a role in appendicitis development. Patients and Methods:  Just 30 (3.15 %) of the 950 surgical specimens removed from patients with clinical appendicitis tested positive for E. vermicularis worms. Two ml blood was drawn from positive samples to measure cytokines (prostaglandin two and Transforming growth factor-beta 1), with 30 healthy people serving as a control group. From January to August 2019, all samples were taken from patients who visited AL-Zahra maternity and paediatrics and AL-Hakeem hospital in AL-Najaf province. Patients' appendixes were biopsies, stained with eosin hematoxylin, and microscopically diagnosed. Results: The findings of this study showed a substantial increase (P0.05) in serum levels of PGE2 and TGF-1β as compared to the control group, as well as a histological spectrum of appendicitis associated with a large number of E. vermicularis eggs, the lymphoid follicle reactive germinal center, prominent eosinophilic infiltration. Conclusion: Based on this research's findings, we believe that E. vermicularis worm infection can cause appendicitis and an immune response manifested by elevated PGE2 and TGF-1β levels in the blood.