Background: Most incidences of cervical cancer are caused by the human papillomavirus (HPV), which can be prevented with a vaccination. In around 90% of cases, HPV infections have no symptoms and disappear on their own within two years. In certain situations, however, an HPV infection might remain and cause warts or precancerous lesions. HPV causes nearly all cervical cancer cases; two strains, HPV16 and HPV18, accounting for 70% of cases. Objectives: The study was designed to re-assess the prevalence of HPV infection among womenattending the GynecologicalOutpatient Clinic Al-Azhar University Hospitals using the new liquid-based cytology technique and our results provide important information for public health authorities considering HPV prevention in Egypt. Subjects and methods: Our study was conducted on 1000 gynecological patients attending Al-Azhar University Hospitals between March 2021 and December 2021. Their ages ranged from 35 - 70 years old. Cervical samples were collected by a gynecologist using a cytobrush and placed in a liquid-based cytology medium (using the DC LBC from GZLBP) for the presence of HPV DNA using Linear Array HPV genotyping at Cytolab Laboratory, Cairo, Egypt. Results Among 1000 women who underwent the LBC test, 97 women were positive for HPV DNA findings (9.7%), while the rest of women 903 were test free (90.3%). 57 cases (58.7%) of positive women showed different degrees of cervical intra-epithelial neoplasia CIN.  Conclusion: The essential need for a proper screening system for cervical pathologies and further multi-central randomized studies are strongly recommended through our study.