Background: Silent information regulator 1 [sirt1] is a protein that is widely expressed and has a crucial function in the prevention of oxidative stress, which is implicated in the development of a number of chronic disorders, such as fatty liver disease.
Objective: The aim of the current work was to investigate SIRT1 gene expression in non-alcoholic fatty liver disease and it is emerging role in pathogenesis of non-alcoholic fatty liver disease. Subjects and Methods: This case-control study included a total of twenty cases of Non-Alcoholic Fatty Liver Disease; NAFLD (Group A) and ten individuals as a control (Group B), attending at Departments of Internal Medicine and Medical Biochemistry, Zagazig University Hospitals. They were assessed clinically by lab investigations and quantitative real time polymerase chain reaction (RT-PCR) to assess Sirt1 gene fold expression.
Results: There was a significant lowering of sirt1 gene expression p=0.0001 for patient with NAFLD compared to healthy controls. There was significant association between SIRT1 and BMI, as well as hyperlipidemia in NAFLD compared to healthy group. We found a significant association between SIRT1 and NAFLD patients with mild fibrosis and those without fibrosis. Cut off value ≤0.61 had 80.0% sensitivity and 80.0% specificity, positive predictive value 57.0%, negative predictive value 92.0% and accuracy was 80.0%.
Conclusion: It could be concluded that the significant correlation between SIRT1 gene expression and non-alcoholic fatty liver disease helps to differentiate patients with nonalcoholic fatty liver diseases and healthy ones.