Background: The febrile seizure is the commonest form of seizure in children. Several hypotheses propose that neurotransmitters and trace elements have a role in the beginning of a febrile seizure. Inhibitory pathways in the central nervous system (CNS) can be affected by zinc, an essential component of many enzymes. Aim and objectives: Thisstudyaimed to determine the relation between serum zinc level and febrile seizures. Subjects and methods: This prospective case control study was carried in Al -Wahda hospital- pediatrics department in Derna city in the north-eastern part of Libya in the period between March 2019 to March 2020. This study comprised 120 children aged between 2 months and 6 years. They were divided to 3 groups: 40 children with febrile seizures, 40 children with fever and no seizures, and 40 healthy children. Results: Zinc levels varied significantly amongst the three groups investigated. The differences between the groups with and without febrile seizures, as well as between febrile seizure groups and the controls, are also notable. The seizure-free group and the controls, on the other hand, showed no considerable differences. Conclusion: According to the current findings, serum levels of zinc were lower in children with febrile seizures. Seizures in febrile children may be facilitated by zinc deficiency.