Background: Needle stick injuries (NSIs) increasing day by day and globally a lot of health care workers suffer from infection per year through this route. Objective: This study was aimed to assess knowledge, attitude, and practice of primary health care workers toward needle stick injury at Fakous district, Sharqia governorate. Patients and Methods: This interventional study included a total of 104 personnel who were working in the of primary health care centers (PHCs), at Fakous district, Sharqia Governorate. This study was conducted between January 2020 to January 2021.  Results: There was highly statistically significant association between history of needle stick injury and history of HBV vaccination as positive history of HBV vaccination (Three doses) was associated with positive history of needle stick injury. There was high statistically significant improvement in knowledge of the study participants about standard precautions of infection control after intervention except for waste disposal. Before intervention, the highest proportion of adequate knowledge was about waste disposal (93.3%) while the lowest proportion of adequate knowledge was about sterile instruments and devices (15.4%). Conclusion: It could be concluded that the effect of health education is remarkable in improving the development of knowledge and increasing awareness of health care providers about infection control guidelines.