Background: Atopic dermatitis (AD) is a very common persistent skin disorder at whichskin colonization by bacteriaincreases. Staphylococcus aureus can be found on the skin as a human commensal or as a causal agent in a variety of skin and soft tissue infections. Objective: This study focused on detecting staph aureus colonization on AD lesions and if it influenced the severity of the disease. Patients and Methods: Swabs from skin lesions of 108 atopic dermatitis patients were collected and subjected for bacterial isolation, identification, and antibiotic susceptibility testing. Results: Among 108 AD patients, Fifty percent of patients had mild AD disease, 37% had moderate disease, and 13% had severe disease. Staph aureus colonization in AD patients was 61.1%. There were no significant differences between positive and negative staph aureus culture groups regarding age (P-value 0.57), sex (P-value 0.38), and the most prominent lesion location (P-value 0.08). There was no significant difference in the severity of AD between positive and negative staph aureus culture groups (P-value 0.09). Methicillin-resistant Staph Aureus was detected phenotypically by cefoxitin (30 µg) disc in 84.8% of isolates. Conclusion: Staphylococcus aureus colonization was detected with a high percentage among atopic dermatitis patients with an extremely higher incidence in severe forms of atopic dermatitis than mild forms. However, this couldn't be proved statistically.