Background: The frequency of malnutrition among hemodialysis (HD) cases differs widely depending on the study participants and assessment methods. Objective: This study aimed to estimation of the prevalence of malnutrition among hemodialysis adult patients in Zagazig University Hospitals by using different methods. Patients and Methods: At Zagazig University Hospitals, Nephrology Unit, Internal Medicine Department, we conducted our study on 95 patients with end-stage renal disease (ESRD) on regular hemodialysis. All patients underwent a thorough history-taking as well as laboratory investigations. Results: Mini-nutritional assessment had a statistically significant relationship with gender, body mass index (BMI), and dialysis duration. The mini-nutritional assessment (MNA) score, hemoglobin, hematocrit, serum iron, serum calcium, serum albumin, and high-density lipoprotein (HDL) cholesterol had a statistically significant positive correlation. Low-density lipoprotein (LDL) cholesterol is negatively correlated with the mini-nutritional assessment score. The correlation between Subjective Global Assessment (SGA) and BMI was statistically significant. Among factors significantly correlated to MNA score, only BMI (unstandardized β=0.978, 0<0.001) and serum albumin unstandardized β=1.625, p=0.004) significantly independently correlated with it. Conclusion: Chronic kidney disease patients who receive hemodialysis on a regular basis are more likely to suffer from malnutrition. Malnutrition becomes more common as people get older and their kidney function deteriorates. The MNA and SGA Score, when used as regular screening tools, revealed a significant risk of malnutrition and can be used during the treatment course and rehabilitation of ESRD patients.