Background: Worldwide, data are conflicting on the prevalence of Helicobacter pylori (Hp) infection in Hemodialysis (HD) population. In Egypt, the data on this population are rather limited. Objectives: The aim of this cross-sectional study was to screen HD patients with Gastrointestinal (GI) symptoms for the prevalence of Hp infection using non-invasive serological testing for Immunoglobulin G (IgG) antibody and then to compare the screening data with the confirmatory esophagogastroduodenoscopy (EGD) findings. Patients and Methods: From January 2020 to June 2021, the sera of one hundred patients with GI complaints were screened for IgG antibody against Hp, among them fifty patients underwent EGD as per indication. Univariate and multivariate analysis were performed to compare serological versus invasive testing performance for Hp infection. Results: In the present study, (60%) of the screened study population demonstrated a positive IgG against Hp, whereas the EGD findings confirmed Hp infection in (50%) of the fifty patients who underwent a confirmatory EGD. In comparison to EDG findings, IgG Antibody sensitivity and specificity for diagnosis of Hp infection was 65.5 % and 52.4 % respectively. Conclusion: Among the one hundred HD patients screened for IgG against Hp, we found a seroprevalence of 60%; however, the performance characteristics of IgG antibody were limited. Further studies are warranted to explore these findings.