Background: Left ventricular ejection fraction (LVEF) is a strong prognostic parameter in patients with heart disease. The assessment of global longitudinal strain (GLS) from speckle-tracking analysis of 2-dimensional echocardiography has become a clinically feasible alternative to LVEF for the measurement of myocardial function. Objective: The aim of the current work was to compare between GLS speckle tracking echocardiography and Simpson's biplane methods for assessment of left ventricular function in non-STEMI patients. Patients and methods: This study was carried out in Cardiology Department, Faculty of Medicine, Zagazig University on 44 non‐ST‐elevation myocardial infarction (NSTEMI) patients scheduled for assessment of Left ventricular function by GLS and biplane Simpson method. Result: Mean systolic blood pressure was 122.05±18.37 mmHg. No statistically significant difference in systolic blood pressure was found across GLS groups (P= 0.17). Mean diastolic blood pressure was 73.41±12.00 mmHg. No statistically significant difference in diastolic blood pressure was found across GLS groups (P= 0.35). Mean heart rate was 68.84±7.13 bpm, and a statistically significant difference was found in heart rate across GLS groups (P= 0.039). Conclusion: It could be concluded that GLS speckle tracking echocardiography and Simpson's biplane methods can be used as alternative different parameters for assessment of left ventricular function in non-STEMI patients.