Background: Chronic liver diseases encompasse metabolic, genetic, drug-induced and inflammatory diseases. The causes of liver disease in children vary with age. In countries where HBV is endemic, perinatal transmission remains the most important cause of chronic infection. The prevalence of hepatitis C infection was much higher in individuals receiving blood products for conditions such as thalassemia or haemophilia. Glycogen storage disorders may present with chronic liver disease. Objectives: To study epidemiological features and laboratory investigations that may affect the incidence in children with chronic liver disease. Patients and methods: Our study included 30 treatment-naïve patients with chronic liver disease of different aetiologies referred to the Department of Pediatrics, Sohag University Hospital. All patients were subjected to history taking, general and local examinations, laboratory investigations and measurement of serum ceruloplasmin. Results: The mean age of children was 10.23 years in case group and 10.75 years in control group, ranging between 5.5 to 15 years. The majority of patients were females (53.3%) and the remained patients were males (46.7%). There was significant difference between patient and control groups as regard total leucocytic count. Also, there were significant differences between the two groups regarding ALT, AST, total bilirubin, direct bilirubin and concentration and high significant differences regarding serum albumin and prothrombin time. Conclusion: Total leucocytic count, AST, ALT, bilirubin, and serum albumin concentration, and prothrombin time are significant investigations to rule out diagnosis and anticipate complications in patients with chronic liver diseases. ALT and PT are early sensitive markers that anticipate liver decompensation.