Background: MicroRNAs (miRNAs) are small non-coding RNA molecules that control the post-transcriptional gene expression. They play a pivotal role in the regulation of important physiological processes. Variations in miRNA genes coding for mature miRNA sequences have been implicated in several diseases, however, its role in type 1 diabetes mellitus (T1DM) is still underestimated. Objective: To investigate the association of miR-196a2 (rs11614913) and miR-146a (rs2910164) polymorphisms with T1DM in Egyptian children and adolescents. Patients and Methods: A case-control study was done on 100 Egyptian children diagnosed with T1DM and additionally, 100 healthy age- and sex-matched subjects non-diabetic (control group) in the Department of Pediatrics and Endocrine and Metabolism Unit, Zagazig University Children Hospital from March 2021 to August 2021. Random blood sugar (RBS), glycosylated hemoglobin (HbA1c) and laboratory assessment for lipid profile were done for all patients. Results: There was no significant difference between CC, CT and TT genotype in age, BMI, sex, onset, and disease duration, HbA1c, TC, LDL or HDL. There was a significant increasing in TG level in TT genotype compared to other genotypes. There was no significant differences between different genotypes in any of the studied parameters. Conclusion: The present study demonstrated that variants rs11614913 T/C and rs2910164 G/C were linked with the risk of T1DM. The data suggested that rs11614913 T/C and rs2910164 G/C could be considered as novel risk factors in the pathogenesis of T1DM in the Egyptian population.