Background: Shoulder overuse injuries represent a common complaint among adolescents. Overuse injuries usually occur as a result of muscles weakness and imbalance between shoulder external (ER) and internal rotation (IR). Muscle tendon units may have elevated risk of overuse injuries in the actively growing child because as the bone lengthens, the muscle-tendons have to stretch to keep up. This relative tightness and related poor flexibility place young athletes at increased risk of muscle-tendon strains, avulsion injuries, and muscle tears. Objective: The aim of this study was to determine the effect of plyometric exercise on shoulder internal rotation for adolescent after overuse injuries. Patients and methods: A total 30 adolescents boy children were enrolled in this study, their age ranged between 15 to 18 years old. The shoulder internal rotation was evaluated by using universal goniometer. The pain intensity was evaluated by using Wong Backer Faces pain scale (WBFS). Results: All children were graded as 4 “hurts little" by WBFS. There was a significant decrease in glenohumeral internal rotation deficit (GIRD) and increase shoulder internal rotation post treatment compared with that pretreatment in the group A and B (p > 0.001). Conclusion: According to results we concluded that there was significant improvement in shoulder internal rotation and decrease in GIRD for adolescents' shoulders after overuse injuries.