Background: Acute exacerbation is a common problem in the usual course of chronic obstructive pulmonary disease (COPD). Infections play a chief role in Acute Exacerbation of Chronic Obstructive Lung Disease (AECOPD) that leads to significant morbidity and mortality. Objective: To assess the bacteriology in COPD exacerbations of hospitalized patients and correlate with clinical and laboratory data of the patients. Patients and Method: a prospective study was conducted to correlate clinical, laboratory data with sputum culture results of 52 patients, admitted to the Pulmonology Department of Buraidah Central Hospital (BCH) with an AECOPD from 1^st January 2018 till 31 August 2018. All collected sputum samples were subjected to standard microbiological procedures. Results: Growth of pathogenic organisms was seen in 37 (71%) of the 52 sputum samples tested. Gram-positive organisms were found in the majority 20 (54 %). Gram negative microbes accounted for 17 (46%). Streptococcus pneumoniae (24.3 %) was found to be the most common bacterial agent responsible for COPD exacerbations, followed by Klebsiella pneumonia 7 (18.9 %), Haemophilus influenzae (H influenzae) and Staphylococcus aureus; each was 5 (13.5%), Streptococcus pyogenes (Streptococcus pyogenes) 4 (10.8%), Moraxella catarrhalis (M catarrhalis), Pseudomonas aeruginosa, and Methicillin-resistant Staphylococcus aureus (MRSA) each was 2 (5.4%) Conclusion: Sputum culture is considered a simple and good diagnostic modality to identify bacteria in AECOPD. Antibiogram would assist in developing a local antibiotic strategy and reducing the appearance of resistance stains.