ABSTRACT Background: Food additives are substances used in food industry in order to improve the food's taste and appearance. Objective: To investigate the adverse effects of mixing some food additives on the biochemical parameters in male albino rats. Materials and Methods: This study was conducted on twenty-four young male albino rats. Animals were divided into four groups (6 each). Group I: Control group < strong>, Group II: administered with fast green and glycine. Group III: administered with fast green and sodium nitrate and Group IV: administered with sodium nitrate and glycine. After 30 days of treatment, blood samples were collected for biochemical parameters. Results: body weight, total protein, albumin and testosterone hormone decreased in all treated groups. Glucose, insulin and HOMA-IR decreased in group received (fast green with glycine). Rats treated with sodium nitrate plus glycine showed a significant increase in glucose level. The liver enzymes and the kidney function tests increased in all treated groups. Rats treated with fast green plus glycine showed significant decrease in the TG and TC while HDL showed significant increase. Rats treated with sodium nitrate plus glycine showed a significant increase in the TG and TC while HDL showed significant decrease. T3 and T4 decreased in rats treated with the mixture of sodium nitrate and glycine while increased in rats treated with the mixture of fast green and glycine. Conclusion: The use of these compounds must be limited as its use leads to real vehement disturbance in biochemical parameter.