Background: Cesarean section is the most performed major abdominal surgery. While cesarean delivery is usually an uncomplicated procedure, up to 20% of patients can experience a complication following cesarean delivery with infectious complications being the most common. Nosocomial infections represent one of the major sources of morbidity and mortality in hospitalized patients around the world. Objective: The aim of the current work was to evaluate if the different scrubbing methods of surgical team before cesarean section by different materials change the rates of post-operative surgical site infection or not. Patient and methods: This randomized controlled trial (RCT) study included a total of 278 pregnant women, attending at Departments of Obstetrics and Gynecology, Menouf General Hospital and Menoufia University Hospitals, during the period of September 2019 till August 2020. Result: there was no statistically significant difference between the studied groups regarding their demographic and clinical data. There was no statistically significant difference between group A and group B regarding offensive odor at day 10 and 15 post-operatively. No offensive odor reported after day 25 or 30 postoperative (p> 0.05). Also, there was no statistically significant difference between group A and group B regarding approximation at day 10, 20, 25 and 30 post-operatively (p> 0.05. There was no statistically significant difference between group A and group B regarding hotness, redness, tenderness, swelling, discharge and offensive odor at day 10, 20, 25 and 30 post-operatively (p> 0.05). Conclusion: It could be concluded that for the increasing rates of CS being performed without a clear medical indication; new practice protocols should be implemented to reduce the rate of cesarean deliveries as CS surgery has a 5–20 times higher risk of post-partum infection as compared to vaginal deliveries.