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112402

Clinical Utility of Enzyme-Linked Immunosorbent Assay for Hepatitis C Core Antigen Quantification in Diagnosis and Monitoring Patients Treated with Direct-Acting Antivirals in A R

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Last updated: 03 Jan 2025

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Abstract

Background: Hepatitis C virus core antigen (HCV-cAg) assay has been proposed as a more economical alternative to HCV RNA detection. Aim of the work: To evaluate the clinical utility of ELISA for HCV-cAg quantification in diagnosis and monitoring treatment outcomes in patients treated with direct acting antivirals (DAAs) for chronic hepatitis C. Patients and Methods: A prospective study on 40 subjects recruited from hepatology department and outpatient clinic in Ain Shams University Hospitals. Group I included 20 patients with positive anti-HCV antibody. Group II included 20 healthy subjects. The patient group received a combination of sofosbuvir 400 mg and daclatasvir 60 mg once daily for 12 weeks. The levels for both HCV-cAg and HCV RNA were evaluated at baseline and 12 weeks after completion of therapy. Results: Baseline HCV-cAg levels showed good correlation with HCV viral load (r=0.808, p < 0.001). A sustained virological response 12 weeks off therapy (SVR12) was achieved in all patients with HCV-cAg levels decreasing significantly at the end of treatment (EOT) (21.5±8.5 vs 5.4±3.63 IU/ml respectively, p < 0.001). The sensitivity and specificity of HCV-cAg in predicting HCV RNA was 100% and 90% respectively at cut-off value >8 IU/ml. Conclusion: ELISA for HCV-cAg can replace the high sensitivity HCV RNA molecular assay to confirm the presence of HCV infection and to monitor treatment outcome.

DOI

10.21608/ejhm.2020.112402

Keywords

Hepatitis C virus, HCV-core antigen, HCV RNA, Direct acting antiviral agents

Authors

First Name

Ahmed ElMetwally

Last Name

Ahmed

MiddleName

-

Affiliation

Gastroenterology and Hepatology Unit, Internal Medicine Department, Faculty of Medicine, Ain Shams University

Email

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City

-

Orcid

-

First Name

Wael Ahmed

Last Name

Yousry

MiddleName

-

Affiliation

Gastroenterology and Hepatology Unit, Internal Medicine Department, Faculty of Medicine, Ain Shams University

Email

-

City

-

Orcid

-

First Name

Ghada Kamal Mohamed

Last Name

Abdel-Halim

MiddleName

-

Affiliation

Gastroenterology and Hepatology Unit, Internal Medicine Department, Faculty of Medicine, Ain Shams University

Email

-

City

-

Orcid

-

First Name

Walaa Mohammad

Last Name

Hashem

MiddleName

-

Affiliation

Gastroenterology and Hepatology Unit, Internal Medicine Department, Faculty of Medicine, Ain Shams University

Email

-

City

-

Orcid

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Volume

81

Article Issue

1

Related Issue

16813

Issue Date

2020-10-01

Receive Date

2020-09-13

Publish Date

2020-10-01

Page Start

1,281

Page End

1,284

Print ISSN

1687-2002

Online ISSN

2090-7125

Article File

EJHM_Volume 81_Issue 1_Pages 1281-1284.pdf

PDF . 443.7kB

Link

https://ejhm.journals.ekb.eg/article_112402.html

Detail API

https://ejhm.journals.ekb.eg/service?article_code=112402

Order

20

Type

Original Article

Type Code

606

Publication Type

Journal

Publication Title

The Egyptian Journal of Hospital Medicine

Publication Link

https://ejhm.journals.ekb.eg/

MainTitle

Clinical Utility of Enzyme-Linked Immunosorbent Assay for Hepatitis C Core Antigen Quantification in Diagnosis and Monitoring Patients Treated with Direct-Acting Antivirals in A Resource Limited Setting

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Type

Article

Created At

22 Jan 2023