Background: Platelet-rich plasma (PRP) is an autologous product derived from whole blood through the process of gradient density centrifugation. Autologous PRP, with growth factors up to 8 times that of normal serum, has been shown to be safe and effective in promoting natural processes of wound healing. Objective: Assessment of the efficacy and advantage in using autologous platelet-rich plasma during myringoplasty in closure of tympanic membrane perforation improving overall outcome. Method: Platelet-rich plasma (PRP) is an autologous platelet-rich concentrate prepared from patients' own blood with growth factors up to 8 times that of normal serum. The efficacy of PRP when used during myringoplasty is studied. 40 patients with chronic otitis media inactive mucosal disease were randomly chosen and 20 of them was the study group and other 20 were the control group. Both group patients underwent myringoplasty and PRP was used in the study group and the results were evaluated. Myringoplasty was done and after placement of the cartilage graft, platelet-rich plasma was placed over the myringoplasty site. Results: In our study among cases that underwent myringoplasty with use of platelet-rich plasma, 90% had complete tympanic membrane closure and only 10% failure has been noticed. In controls 60% out of cases had failure. The graft take up rate in our study is comparable with the reference studies. Use of PRP accelerated graft uptake. Conclusion: Platelet-rich plasma is a cheap and cost effective platelet concentrate with enriched growth factors. It accelerated the tympanic membrane closure following myringoplasty.