Background: Various methods are used for gender identification in anthropologic and forensic investigations. The canine tooth are widely used for gender determination because of its sexual dimorphism and durability in oral cavity. Mandibular canine index is one of the most reliable and valid predictor for gender identification. Aim: To assess the reliability of Mandibular Canine Index in gender determination in North Saudi Sakaka populations. Settings and Design: This was an experimental study involving the preparation of plaster cast models on 100 individuals residing in North Saudi Sakaka populations. Out of these 55 were males and 45 were females. Both sexes were in the age group of 20- 40 years old. Study casts were prepared for all individuals and the measurements of mandibular canine teeth were taken. The Mandibular canine index was calculated using standardized equation. In addition, the percentage of sexual dimorphism was calculated. Independent sample t test was used. P value < 0.05 was considered statistically significant. Results: There was a significant difference (p value < 0.05) observed between intercanine distance, right and left canine width and right and left mandibular canine index between males and females. Sexual dimorphism was more on left permanent mandibular canine teeth than right permanent mandibular canine teeth. The overall percentage of accuracy observed was 85.5 %. Conclusions: Study findings revealed that mandibular canine index can be used as a reliable predictor for gender determination in Sakaka population and can form the basis of anthropologic and forensic investigations.