Background: Dental caries remains to be the most common oral disease, caused by the unbalance between demineralization effect of bacteria and remineralization process. In the early phase of caries, this balance can be restored and the demineralization effect reversed using an enhanced approach to remineralization. Objective: The aim of the study is directed to evaluate the degree of demineralization and remineralization in subclinical carious lesion using chitosan and conventional remineralizing agent. Materials and methods: A total number of 40 sound non-carious human anterior teeth were collected from oral surgery clinic, free of cracks and any developmental defects, were used in this study in age range (20-40) years old. Teeth were stored in distilled water at room temperature until use. The teeth were divided into four main groups (10 teeth each). Results: In the present study specimens treated with chitosan after six months storage time (the best result) recorded the highest mean value of remineralization while fluoride record the lowest mean value of remineralization (the worst result) through the remineralizing agents chitosan, casein, and fluoride. The specimens subjected to Diagnodent fluorescent reading proved that for all remineralizing agents (chitosan, casein and fluoride) and also control group the remineralization to tooth structure was increased by increasing the storage time. Conclusion: Chitosan is considered a valuable biomimetic remineralizing agent. Mineral precipitation to tooth structures by using remineralizing agents is a continuous dynamic process.