Background: Three-dimensional echocardiography (3D-Echo) has been incorporated into routine clinical practice in many echocardiography laboratories, as it provides many advantages in the evaluation and study of heart diseases. Objective: To estimate the maximum normal systolic reference value of AS in a sample of healthy subjects. Patients and methods: This study was conducted in the Islamic Cardiology Centre in Al Azhar University, Real Time 3D Speckle Tracking Echo data set was acquired over 24 months in 90 healthy subjects divided into 3 main groups according to their age: 1- First Group 16-30 years old, 2- Second Group 31-50 years old and 3-Third Group 51-65 years old. Results: II and age group III were -22.07 ± 1.48%, -19.40 ± 1.98% and -17.67 ± 1.52% respectively. Our study showed that the mean Global Longitudinal Strain at age group I, age group II and age group III were -19.2 ± 1.52%, -18.9 ± 1.96% and -17 ± 0.99% respectively. The present study showed that the mean Global Area Strain at age group I, age group II and age group III were -29. 1±1.31%, -28.5 ± 1.76% and -28.1 ± 1.65% respectively. Our present study showed excellent intraobserver reproducibility of the results and an overall acceptable interobserver reproducibility of strain measurements. Conclusion: Global area strain derived from three-dimensional speckle-tracking may be applied clinically as a supplementary diagnostic method since strain imaging can identify LV dysfunction earlier than conventional methods. This opens a new perspective in heart failure prophylaxis and primary prevention with institution of therapeutic measures before the patients develop symptoms and irreversible myocardial dysfunction.