Beta
47983

In Vitro Evaluation of Antiviral / Virucidal Activity of Naja Nubiae (Elapidae) Venom Against Rift Valley Fever and Herpes Simplex Virus Type -1 (HSV-1) Using Cell Culture

Article

Last updated: 03 Jan 2025

Subjects

-

Tags

-

Abstract

Background: Venoms are pretentious products secreted by snake, invertebrates and scorpions. Venoms composed of different proteins, toxic fractions, enzymes and hormones. Some fractions proved bioactivity including anticoagulation. antimicrobial and anticancer activity. Objective: The aim of the current study was to in vitro evaluate the Antiviral / virucidal activity of Naja nubia (Elapidae) venom against Rift Valley Fever (RVFv) and Herpes simplex virus type -1 (HSV-1) using cell culture. Materials and methods: Toxicity of tested venom was performed using cell culture to determine the safe concentration using MTT assay. Antiviral activity was conducted by precultured cell treatment with safe concentration of snake venom for 24 hr. then virus was dispensed as 0.1 ml / well in quadrate for each dilution. TCID50s of snake venom safe concentration treated and untreated cells were determined. The difference is the antiviral activity. Virucidal was conducted by mixing one part of virus models with nine parts of the safe concentration of snake venom at 37oC. The infectivity titer / time interval was determined according to Reed and Menuch equation. Result: Data record revealed that the cell viability was concentration dependent recording an IC50 of 9.3 µg/ ml. Also, venom showed a very limited antiviral activity but the virucidal activity was time dependent and showed a depletion rate in the order of 0.72 log (10) and 0.94 log (10) /hr. for RVFV and HSV-1 respectively indicating that RVFv was moderately sensitive than HSV-1. Conclusion: It could be concluded that Naja Nubiae venom is a potentially virucidal agent than antiviral one with persistent inactivation potential.

DOI

10.21608/ejhm.2019.47983

Keywords

Naja nubiae, Antiviral, Virucidal, Rift valley forever virus, Herpes simplex type 1, cell culture, MTT

Authors

First Name

Kareem A.

Last Name

Yonys

MiddleName

-

Affiliation

Department of Zoology, Faculty of Science (Boys branch), Al-Azhar University

Email

karemyonys1990@gmail.com

City

-

Orcid

-

First Name

Samy A.

Last Name

Saber

MiddleName

-

Affiliation

Department of Zoology, Faculty of Science (Boys branch), Al-Azhar University

Email

-

City

-

Orcid

-

First Name

Abir A.

Last Name

El-Fiky

MiddleName

-

Affiliation

ANDI COE in Antivenom Research

Email

-

City

-

Orcid

-

First Name

Aly F.

Last Name

Mohamed

MiddleName

-

Affiliation

R&D Sector VACSERA

Email

-

City

-

Orcid

-

Volume

77

Article Issue

2

Related Issue

7349

Issue Date

2019-10-01

Receive Date

2019-09-13

Publish Date

2019-10-01

Page Start

4,963

Page End

4,969

Print ISSN

1687-2002

Online ISSN

2090-7125

Link

https://ejhm.journals.ekb.eg/article_47983.html

Detail API

https://ejhm.journals.ekb.eg/service?article_code=47983

Order

14

Type

Original Article

Type Code

606

Publication Type

Journal

Publication Title

The Egyptian Journal of Hospital Medicine

Publication Link

https://ejhm.journals.ekb.eg/

MainTitle

In Vitro Evaluation of Antiviral / Virucidal Activity of Naja Nubiae (Elapidae) Venom Against Rift Valley Fever and Herpes Simplex Virus Type -1 (HSV-1) Using Cell Culture

Details

Type

Article

Created At

22 Jan 2023