Background: A successful in vitro fertilization treatment depends on the ability of the ovary to respond to gonadotrophin stimulation. This response reflects the ovarian function or ‘ovarian reserve'. Poor ovarian response may be associated with reduced number of oocytes and embryos and may lead to cycle cancellation in severe cases. On the other hand, exaggerated response leads to increased risk of ovarian hyper-stimulation syndrome. Objective: The aim of this study is to assess role of serum AMH, FSH and AFC measurement as a prediction of pregnancy rates in IVF/intracytoplasmic sperm injection (ICSI) cycle. Patients and Methods: A prospective cross sectional study was conducted at Specialized Air Forced Hospital in the period between January 2018 and December 2018. The study included 100 unexplained infertility patients undergoing IVF/ICSI treatment cycles. They were 82 patients primary infertility and 18 patients secondary infertility. Results: Type of infertility showed insignificant difference between the 2 groups while menstrual history and gonadotrophins type showed significant difference. Age and duration of infertility were significantly higher in nonpregnant group than pregnant. The mean value of FSH, day 3 E2 levels did not have any significant difference between the 2 group while the mean value of serum E2 the day of HCG injection, serum AMH day 3, AFC were significantly higher in pregnant group than non-pregnant. Conclusion: No test can predict pregnancy in high accuracy as it is multifactorial however, mean levels of FSH, E2, a day HCG injection and AFC showed significant difference between pregnant and non-pregnant.