Background: The identification of sex from human remains is of fundamental importance in forensic medicine and anthropology, especially in criminal investigations as well as in the identification of missing persons and in attempts at reconstructing the lives of ancient populations. Objective: The aim of this study was to assess specific pelvic parameters (Human sacrum and subpubic angle measurements) in Assiut Governorate population by using plain x-ray films. Patients and methods: The study was conducted on 500 plain x-ray films, which were obtained and collected from the Radiology Department- Al-Azhar Faculty of Medicine (Assiut branch), out of which, 250 were males and 250 were females in the age group of 25–70 years belonging to Assiut population to assess the sacral and subpubic angle measurements. Results: Statistically significant increase in the length of sacrum in males compared to females. The sacral length in males varied from 86–120 mm with the mean value of 100.06 ± 9.00 mm, while the sacral length in females varied from 78–120 mm with the mean value of 93.00 ± 9.46 mm. Conclusion: The present study concluded that the subpubic angle is the best criterion for sex determination of pelvis. It was found that 66.4% of males' bones and 58.4% of females' bones could be identified by using subpubic angle alone.