Background: uterine fibroid (leiomyoma) is the most common benign uterine tumors in women of reproductive age. The majority of leiomyomas are asymptomatic, however up to 20% cause menorrhagia, pelvic pain and genitourinary symptoms. Objective: the aim of the study was to evaluate using of total protein or serum prolactin as a marker for evaluation and follow up of uterine leiomyomas. Patients and Methods: a randomized, controlled clinical trial study design was utilized to prove the relation between uterine fibroid and serum prolactin and serum protein. The study was performed at Obstetrics and Gynecology Department in AL Hussein Hospital. 100 females have myoma of different sizes are included in this research. Results: it was concluded that serum total protein and albumin levels in patients with elevated serum TNF levels were significantly lower (p < 0.05) than the corresponding values in patients with undetectable serum TNF levels. The study concluded that prolactin (PRL) levels are decreased following surgical therapy in patients with uterine fibroids. In addition, a slight increase in serum total protein was detected after surgery in uterine patients. Conclusion: in conclusion, a highly significant correlation was found between serum PRL and its ratio with serum total protein. This result ensured and supported the relation between the patient's serum PRL and serum total protein with their fibroid number. This enables to suggest these variables as a simple biochemical marker supporting other clinical findings.