Background: Food additives are substances intentionally added to food to change its characteristics, maintain and improve safety, maintain the nutrient value and to improve taste, texture, and appearance. Aim of the work: This study aimed to determine the therapeutic effects of vitamin C against the hazardous effects of sodium benzoate, chlorophyllin and monosodium glutamate on some physiological parameters in male albino rats. Materials and Methods: this study had been done on thirty male albino rats with an average body weight 120-140 g. The animals were divided into three groups. Group 1: control, Group 2: rats treated with mixture sodium benzoate, chlorophyllin and monosodium glutamate and Group 3: rats treated with food additives mixture and vitamin C. Blood samples were collected and the separated sera were used for estimation of some biochemical parameters (liver enzymes, kidney functions, glucose, protein profile and lipid profile) and hormonal levels [testosterone, triiodothyronine (T3) and thyroxine (T4)]. Results: The biochemical results showed an increase in levels of fasting glucose, insulin, HOMA-IR, activities of AST and ALT, urea, creatinine, total cholesterol (TC), triglycerides (TG), LDL-C, VLDL, ratios of TC/HDL-C and LDL-C/HDL-C (risk factors) in mixture group accomphined by a significant decrease in protein profile (total protein, albumin and globulin), HDL-and testosterone hormone levels . On the other hand, these results turned back nearly to normal values after receiving vitamin C. Conclusion: The present study revealed capability of vitamin C to fight the grievous effects of food additives mixture on major physiological parameters.