Background: LV diastolic dysfunction is one of the first signs of diabetic cardiomyopathy, often developing before systolic dysfunction. Diastolic dysfunction in women differs in many aspects from that of men. Some of these differences may have a pathophysiological basis. These sex differences may have widespread implications in the field of heart failure with normal ejection fraction. Objective: To assess gender differences of left ventricular diastolic function by pulsed and tissue Doppler echocardiographic indices in patients with type II diabetes mellitus. Patients and Methods: The study included 300 patients with type II diabetes mellitus and was conducted in the Cardiology Department (Faculty of Medicine, Azhar University). It is an observational case control study started from January 2017 to March 2019. 150 age and gender matched healthy volunteers as control. Results: Our study showed that there is no statistically significant difference between diabetic males and diabetic females as regard LA dimensions, E/é lateral, e' lateral, E/A ratio, A velocity. It was significant for E/e' septal, E velocity, EF, LVEDD and LVESD with p values 0.033, 0.006, 0.001, 0.007 and 0.001 respectively. Conclusion: Diabetic patients should be evaluated for subclinical diastolic dysfunction by Doppler studies as well as good control of diabetes for deceleration of the development of clinical cardiomyopathy, and decreased morbidity and mortality.