Background: Süßstofftabletten is an artificial sweetener, which is mainly composed of cyclamate and saccharin. Aim of the work: clarification of Süßstofftabletten effects and the amelioration effect of vitamin E in male albino rats. Materials and methods: thirty male albino rats weighing from 100 to 120 gm. The period of the experiment was 30 days. The animals were divided into three groups; group 1: control, group 2: rats received Süßstofftabletten (1 tablet/25kg b.w./day) and group 3: rats received Süßstofftabletten (1tablet/25kg b.w./day) + vitamin E (6 mg/kg b.w./day). The following parameters were measured: serum glucose, ASAT, ALAT, serum creatinine, serum urea, protein and lipid profiles and hormonal levels (insulin, testosterone, serum T3 and serum T4). Results: there were many disturbances that occurred in the previous parameters, and vitamin E ameliorated most of these hazardous effects. Conclusion: the assertions that artificial sweeteners are safe and healthy in use by consumers are false. Natural sweeteners whose benefits are important and necessary to our bodies must be used instead of artificial sweeteners. Also vitamin E is very important in our daily diet as it fights many damages that encounter us.