Background: Phytoestrogen is a plant-derived compound, which has estrogenic effect and it is found in liquorice root extract and sesame oil.Aim of the work:To investigate some biochemical effects of liquorice root extract and sesame oil on male albino rats. Materials and methods: 18 animals were divided randomly into three groups. Group A: Control group, group B: rats treated with oral dose of liquorice10 mg/kg body weight/day for one month, andgroup C: rats treated with oral dose of sesame oil 1 ml/kg body weight/day. At the end of the experiment, blood samples were collected for biochemical analysis. Results: Liquorice and sesame oil induced highly significant decrease in TC, TG, LDL, VLDL, LDL/HDL(p<0.01)and significant increase in HDL(p<0.05). They also showedhighly significantly decrease in FSH, testosterone and sperm countcompared to control group(p<0.01). Conclusion:The study supports that the high level intake of liquoriceroot extract orsesame oil caused hormonal disturbance and decreases sperm count.