Purpose: due to introduction of femtosecond Laser technology as new tool in penetrating keratoplasty (PKP), this study aims to compare this new tool and conventional PKP, also aims to compare between different shapes of femtosecond Laser (FSL) assisted PKP. Patients and methods: 40 eyes with different corneal pathologies were included in this study and they were categorized in to 2 groups. 20 eyes underwent penetrating keratoplasty by using manual trephination (I) and 20 eyes underwent PKP by using FSL assisted trephination (II). Results: femtosecond Laser assisted penetrating keratoplasty (FLAK) substantially improved wound healing, speeds up visual recovery and improved BCVA as it decreased MRA and corneal steepening but consumed more operative time. Conclusion: FLAK is the future in corneal graft surgery, where healing may not be a problem in the postoperative period