Background: it is suggested that vitamin D status is associated with androgenic profile in women with PCOS. Although there are a lot of clinical trials known in this regard, the results were varying. Objective: This study aimed to evaluate the effect of Vitamin D supplementation on androgenic levels (including serum total testosterone, free testosterone and sex hormone binding globulins) in adult women with PCOS were established by the presence of hyperandrogenemia or clinical hyperandrogenism. Patents and Methods: in the present study, 50 outpatient clinic women were included and divided into two groups, first group consisted of 25 patients who received 4000 IU daily of Vitamin D3 for 6 months, and the other group consisted also of 25 patients who received placebo drug blindly. Results: the results showed significant difference between the two groups regarding different laboratory tests especially total testosterone level (decreased significantly after vit. D intake) and SHBG (elevated significantly after vit. D intake) and also significant difference in the hirsutism state after vitamin D administration. But in contrast, there was no significant difference between free testosterone level before and after vit. D administration. These results can help in the confirmation of the suggestion that vitamin D intake can help significantly in decreasing the androgenic profile in a woman patient with PCOS and also in decreasing hirsutism symptom. Conclusion: vitamin D intake can help significantly in decreasing the androgenic profile and hirsutism in a woman patient with PCOS and used as a proper treatment for them.