Background: Intracytoplasmic sperm injection (ICSI) is an in vitro fertilization procedure in which a single sperm is injected into an oocyte. Objective: Aim of the Work:   Comparing three-dimensional ultrasound parameters, with anti-Mullerian hormone (AMH) and other conventional endocrine markers for the prediction of ovarian response in patients undergoing intracytoplasmic sperm injection cycles. Methods: This observational prospective study was conducted at Alexandria Armed Military Hospital, on a sample of 100 women aged between 25 and 35 years, enrolled for intracytoplasmic sperm injection (ICSI). Results: a good correlation between inhibin B and other numerical variables. There was a strong positive correlation between inhibin B and AMH, strong negative correlation between inhibin B and both FSH and LH. Inhibin B correlated moderately negatively with age and moderately positively with AFC, number of collected oocytes. Inhibin B correlated positively with the number of oocytes and number of grade 1 embryos. As regard pregnancy rate, the good-response group had significantly higher chemical and clinical pregnancy rates, as there was no chemical or clinical pregnancy occurred in poor responders. Conclusion: AFC, AMH and Inhibin B are effective in predicting the ovarian reserve as well as the response to induction, and both AFC and AMH are the most significant predictors of response to ovarian stimulation during ICSI. Recommendations: Larger scale studies including higher number of patients are needed to verify the validity of this parameter as a marker of ovarian reserve in patients undergoing ICSI.