Background: acute uncomplicated cystitis (AUC) in females is among the most frequent infections in community. Treatment of AUC in Egypt usually based on empirical therapy. Objectives: To determine the most common uropathogens and their pattern of resistance to commonly used antimicrobials aiming at finding a proper antibiotic which can be used as effective empirical therapy in AUC. Patients and Methods: Observational, hospital based, cross sectional study was performed. Three hundred and twelve female patients were recruited for the study with positive urine culture and clinical symptoms correlate with AUC during the period from December 2017 to August 2018. Results: Eight organisms were isolated. E. coli was the most common uropathogen and was isolated from 231 cases (74%). The next most common was Pseudomonas which was detected in 41 cases (13.1%). Other organisms include MRSA (6.1%), Klebsiella (2.2%), Enterococcus spp. (1.9%), proteus (1.3%), Acinetobacter and Staph. aureus (0.6%) each. Isolated uropathogens in this study were least resistant to Nitrofurantoin (47.1%). The highest resistance rate was to TMP/SMZ (87.8%). Resistance pattern to quinolones were 64.1%, 67% and 60.6% to Ciprofloxacin, Norfloxacinand and Levofloxacin respectively. Conclusion: The most common cause of community acquired AUC was E. coli. Nitrofurantoin can be used as a first line empirical therapy for AUC as it showed the least resistance pattern (47.1%). Antimicrobial resistance is a crucial issue which evolves continuously and thus more studies must be carried out in different regions and constanttime intervals.