Background: Refractive surgery, particularly laser in-situ keratomileusis (LASIK)surgery, at present offers hope in pediatric anisometropia, particularly where traditional therapy has failed. Studies show that it is an effective and safe procedure that corrects high anisometropia and improved binocularity, when conventional therapies had failed. The aim of the Work: to evaluate the visual outcomes of patients with anisometropic amblyopia after laser in-situ keratomileusis (LASIK) surgery. Patients and Methods: This study enrolled 20 anisometropic eyes of 20 patients ranging from 6 to 12 years with refractive difference between two eyes more than four diopters, LASIK was performed in the amblyopic eye under topical or general anaesthesia according to the cooperation of the patients. Visual acuity, refraction, corneal topography, and complications were evaluated. Results: The mean preoperative spherical equivalent refraction (SE) in the operated eye was -5.89±1.46 diopter (range: -10.00 to - 4.24D), significantly decreased down to -0.36±0.19 D at one week, -0.38±0.19 D at one month, 0.46±0.26 D at three months postoperatively. The mean uncorrected visual acuity in the operated eye was 0.58±0.13 (range: 0.30_A0.8) gained five or more lines on snellens chart. The best corrected visual acuity also shows a great improvement in comparing to the preoperative state by one or more lines three months after surgery. Conclusion: LASIK is effective, safe and predictable alternative method for correcting anisometropia in children.