Background: Hormonal control for multiple follicular growths in IVF program was directed toward maximizing the yield of fertilizable oocytes, enhance successful implantation and improve early embryonic development. Aim: The effect of different doses of urinary human menopausal gonadotropin (U-hMG) in superovulation regimen was studied. Materials and Methods: Four groups of immature female mouse were sequentially injected by 5 IU, 10 IU, 15 IU and 20 IU of U-hMG followed by injection of 15 IU human chorionic gonadotropin (hCG) for all groups. Mature cumulus oocytes complexes (COCs) were collected 12 hours later then the grading of cumulus cell expansion and oocytes maturity was determined. In vitro fertilization for mature oocytes was done by using epididymal sperm, the embryonic development was followed up till the blastocyst stage. Results: Results demonstrated that the super ovulatory response in immature mice increased with increasing dose of hMG. 15 IU U-hMG was the optimal dose that gave maximum number oocytes with higher maturation rate,minimum degeneration rate and support embryonic development. Conclusion: 15 IU is the optimal dose for induction of superovulation and support in vitro embryo developmentin immature female mice.