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18739

Ultrastructural and histological changes induced by ivermectin in the ovary of Argas persicus after feeding

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Last updated: 30 Jan 2023

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Abstract

The ovarian wall of A.persicus consists of primary oocytes of three developmental stages namely , young , previtellogenic and vitellogenic in addition to interstitial cells . After feeding and mating , the three stages and interstitial cells , particularly funicle cells that carry oocytes , markedly increased in size and their cytoplasmic organelles exhibit notable changes correlated with yolk and egg shell formation . The present study examined the hitological and ultrastructural aspects during the formation of yolk and egg shell. The first seem to originate from small vesicles derived from Golgi bodies, rough endoplasmic reticulum and mitochondrial cristae. The egg shell seems to develop from vesicles derived from Golgi bodies that fuse with the cell membrane and discharge their content into the extracellular space between basement membrane and tunica propria. Glycogen aggregates and lipid droplets are commonly observed between yolk spheres. The injection of selected dose of ivermectin (400 ug/kg) prevented growth and development of oocytes. The ovary appeared studded with young and previtellogenic primary oocytes surrounding a narrow ovarian lumen or the ovarian wall, carrying oocytes, is stretched around a large fluid filled lumen. Surface microvilli of primary oocytes and coated vesicles underlying them became comparatively fewer. Mitochondria and rough endoplasmic reticulum became fewer, fragmented and finally degenerated in primary oocytes and funicle cells. Ivermectin interfered with the formation of yolk granules and egg shell. However, in the few formed vitellogenic oocytes, Golgi bodies were not affected. The egg shell appeared thinner with low electron density. The cytoplasm of funicle cells became highly vacuolated, its organelles, hardly distinguished and nuclei became swollen without definite nuclear membrane. Ivermectin completely prevented the formation of glycogen particles. Our results suggests that ivermectin causes partial blockage of the release of the vitellogenetic hormone that regulates vitellogenesis and other aspects of oogenesis as indicated by disrupted synthesis and uptake of yolk protein in the developing oocytes.

DOI

10.21608/ejhm.2003.18739

Authors

First Name

Hamdy H.

Last Name

Swelim

MiddleName

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Affiliation

Zoology department Faculty of science, Ain Shams University

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Orcid

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First Name

Aleya S.

Last Name

Marzouk

MiddleName

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Affiliation

Zoology department Faculty of science, Ain Shams University

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Orcid

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First Name

Ashraf A.M.

Last Name

Montasser

MiddleName

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Affiliation

Zoology department Faculty of science, Ain Shams University

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Volume

10

Article Issue

1

Related Issue

3799

Issue Date

2003-01-01

Receive Date

2018-11-11

Publish Date

2003-01-01

Page Start

145

Page End

172

Print ISSN

1687-2002

Online ISSN

2090-7125

Link

https://ejhm.journals.ekb.eg/article_18739.html

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https://ejhm.journals.ekb.eg/service?article_code=18739

Order

10

Type

Original Article

Type Code

606

Publication Type

Journal

Publication Title

The Egyptian Journal of Hospital Medicine

Publication Link

https://ejhm.journals.ekb.eg/

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Article

Created At

22 Jan 2023