Beta
ManageSearchChatDiscover
18224

Immunoprophylactic effect of single and mixed schistosomal antigens on Schistosoma mansoni infected mice

Article

Last updated: 30 Jan 2023

OverviewSimilar Items

Subjects

-

Tags

-

Abstract

B This study aimed to detect the cellular, humoral immune responses and protection against schistosomes induced by cercarial (CAP), soluble worm (SWAP), soluble egg (SEA), and mixed (SEA + CAP), (SEA + SWAP) and (CAP + SWAP) antigens to Schistosoma mansoni infection in mice, and the improvement in the liver enzyme activities before and after challenge with S. mansoni. Each mouse was sensitized with an initial immunization of 0.6 ml of the extracted antigen (30μg protein/ml). After one week, a second injection of 0.4 ml was given (20μg protein/ml). Then, each mouse was exposed to 80 cercariae. Six weeks post challenge the protection percentage was 42.5, 58.33, 53.33, 60.91, 78.16 and 64.77% for CAP, SEA, SWAP, (SEA +CAP), (SEA+SWAP) and (CAP + SWAP), respectively. The results revealed a high significant interrelation between number of lymph node cells (MLN) (P0.001), splenocytes (P0.04) and thymocytes (P0.001) that obtained with each immunized group compared to controls. A high significant difference (P0.001) between levels of IgG obtained between different antigen groups and the control before and after challenge. The immunization with previous antigens resulted in a remarkable improvement in the liver enzyme activities, which were disturbed after infection. Thus, vaccination of mice with the previous different antigens has an immunoprophylactic effect and may protect liver against infection through reduction in worm burden leading to the improvement of many liver enzymes.

DOI

10.21608/ejhm.2004.18224

Keywords

Liver, Mesenteric lymph nodes, Schistosoma mansoni, Splenocytes, Thymocytes

Authors

First Name

Ameen A.

Last Name

Ashour

MiddleName

-

Affiliation

Zoology Department, Ain-Shams University, Cairo, Egypt.

Email

-

City

-

Orcid

-

First Name

Samia A.

Last Name

Ahmed

MiddleName

-

Affiliation

Therapeutical Chemistry Department, National Research Centre , Cairo, Egypt

Email

-

City

-

Orcid

-

First Name

Maghraby

Last Name

A.S

MiddleName

-

Affiliation

Therapeutical Chemistry Department, National Research Centre , Cairo, Egypt

Email

-

City

-

Orcid

-

First Name

Zahran

Last Name

H. G

MiddleName

-

Affiliation

Therapeutical Chemistry Department, National Research Centre , Cairo, Egypt

Email

-

City

-

Orcid

-

Volume

14

Article Issue

1

Related Issue

3783

Issue Date

2004-01-01

Receive Date

2018-11-02

Publish Date

2004-01-01

Page Start

86

Page End

103

Print ISSN

1687-2002

Online ISSN

2090-7125

Article File

EJHM_Volume 14_Issue 1_Pages 86-103.pdf

PDF . 411.9kB

Link

https://ejhm.journals.ekb.eg/article_18224.html

Detail API

https://ejhm.journals.ekb.eg/service?article_code=18224

Order

9

Type

Original Article

Type Code

606

Publication Type

Journal

Publication Title

The Egyptian Journal of Hospital Medicine

Publication Link

https://ejhm.journals.ekb.eg/

MainTitle

-

Details

Type

Article

Created At

22 Jan 2023