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18182

Protective Effect of Allium sativum and Nigella sativa Against NO-Mediated Alterations in Dimethylhydrazine- Induced Colon Cancer rats.

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Last updated: 30 Jan 2023

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Abstract

Plasma levels of nitric oxide (NO) and α-tocopherol as well as catalase activities in colon and liver tissues were assessed in 1, 2 dimethylhydrazine-induced colon cancer rats. Five groups of male Sprague-Dawley rats were fed the experimental diets supplemented with Allium sativum powder and Nigella sativa seeds (2.5%, 5%) or a mixed dose of both plants (5% of each) for 24 weeks, experimental period. At the fifth week rats were subcutaneously injected with dimethylhydrazine dihydrochloride (DMH) at a dose of 20mg/kg body weight for 20 weeks. Another two groups of rats were fed the basal diet for the same period, the first group designed as negative control group and injected with saline solution while the second group was injected with DMH at the same dose and designed as positive control group. Colon carcinogenesis was accompanied by a significant increase in the level of NO as well as catalase activity and significant decrease in plasma levels of α-tocopherol. Only the 5% Allium sativum powder fed group exhibited a significant decrease in NO level. Administration of Allium sativum powder and the mixed dose caused significant decrease in colonic and hepatic catalase activities and significant increase in α-tocopherol levels. On the other hand, the effects of Nigella sativa seeds on the measured parameters were non significant. These results were confirmed by the histopathological results that showed low incidence of colon tumors in rats fed 5% Allium sativum powder (17%) and the mixed dose (56%) fed groups. It could be concluded that the promising effect of garlic in DMH-induced colon cancer rats may be mediated through modulation of plasma levels of nitric oxide and α-tocopherol as well as tissue catalase activity.  

DOI

10.21608/ejhm.2004.18182

Keywords

Colon carcinogenesis, DMH, histopathology, Allium sativum (garlic), Nigella Sativa, Nitric oxide, α-Tocopherol, catalase, rat

Authors

First Name

El-Sayed H.

Last Name

El-Tamany

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Affiliation

Chemistry Department, Faculty of Science, Suez Canal University, Ismailia, Egypt.

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First Name

Awatif M.

Last Name

Abd El-Maksoud

MiddleName

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Affiliation

Clinical Nutrition Department ,National Nutrition Institute, Cairo, Egypt.

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First Name

Ehsan H.

Last Name

Hassan

MiddleName

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Affiliation

Pathology. Departement ,National Hepatology and Tropical Medicine Reasearch Institute, Cairo, Egypt.

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First Name

Abd El-Aziz M.

Last Name

Abd El-Galil

MiddleName

-

Affiliation

Nutrional Biochemistry Department , National Nutrition Institute, , Cairo, Egypt.

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First Name

Shawkia S.

Last Name

El-Sherbiny

MiddleName

-

Affiliation

Nutrional Biochemistry Department , National Nutrition Institute, , Cairo, Egypt.

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First Name

Ismail M.

Last Name

Abdel-Nabi

MiddleName

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Affiliation

Zoology Department, Faculty of Science, Suez Canal University, Ismailia, Egypt.

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Volume

15

Article Issue

1

Related Issue

3782

Issue Date

2004-04-01

Receive Date

2018-11-01

Publish Date

2004-04-01

Page Start

1

Page End

14

Print ISSN

1687-2002

Online ISSN

2090-7125

Article File

EJHM_Volume 15_Issue 1_Pages 1-14.pdf

PDF . 1.3MB

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https://ejhm.journals.ekb.eg/article_18182.html

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https://ejhm.journals.ekb.eg/service?article_code=18182

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Original Article

Type Code

606

Publication Type

Journal

Publication Title

The Egyptian Journal of Hospital Medicine

Publication Link

https://ejhm.journals.ekb.eg/

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Created At

22 Jan 2023