The purpose of this study was to characterize intestinal permeability changes over a range of physiologically relevant intestinal injury. The experiments were performed in 80 rats subdivided into four groups as aspirin (400 mg/kg b.w.), gum Acacia ( 1g./day) and aspirin with gum Acacia groups for 21 days compared with control group. Relative reabsorption of L- Histidine was greater(p<0.001) in the aspirin in 10 min of incubation compared with that of the control rats. In aspirin in combination with gum Acacia, the relative reabsorption were significantly (p<0.001) decrease in 10, 20 and 30 min. of incubation compared with that of the control rats. Moreover, the relative reabsorption of L-histidine was significantly (p<0.01) reduced by the aspirin at 45 min of time of the incubation buffer compared with that of the control. However, gum acacia treatment was increased at 10 min (p<0.01) ,30 min (p<0.01) and 45 min (p<0.001) respectively compared with that of the control rats. Relative reabsorption of L-histidine record a nonsignificant increase of aspirin at 20 min and 30 min of incubation compared with that of the control. Gum and aspirin with gum at 20min and 45min of incubation resulted an increase and decrease in relative reabsorption of L-histidine respectively compared with that of the control. Aspirin and aspirin in combination with gum acacia treatment increased body, intestinal weights and mucosal total protein significantly with percent changes ranged from 8% to 40% compared with that of the control. On the other hand, gum treatment decreased body, intestinal weights and mucosal total protein significantly with percent changes ranged from 8% to 35% compared with that of the control. These results demonstrated that L-histidine is actively taken up by a gum Acacia system in intestinal everted sac mechanism of rat with energy supplied by glucose and Na+in incubation buffer. Moreover, aspirin system had an inhibitory effect on L-histidine uptake in 45 min of incubation , indicating the saturation by L-histidine in first ten minuts of incubation. Also, these results provide evidence the uptake of L-histidine into rat intestine was not reduced at all by the treatment of aspirin. These results suggest that the uptake of L-histidine by intestinal everted sac of rat has different characteristics of aspirin with gum compared with that of the control in respect to relative reabsorption of L- histidine.