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18040

Molecular Biological Studies on the Effect of the Electromagnetic Fields on ETS-1 Oncogene

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Last updated: 03 Jan 2025

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Abstract

ETS-1 is the founding member of the ETS family of transcription factors. ETS factors have important roles in oncogenesis, signal transduction and development. In human tumors, ETS-1 is expressed in endothelial cells and fibroblasts of the tumor stroma and is proposed to play a role in tumor vascularization and invasion by upregulating expression of matrix- degrading proteases. In human carcinomas, ETS-1 is also expressed by neoplastic cells, but little is known about the functional implications of this observation. The present study aimed to detect the tumor by using electromagnetic fields through ETS-1 oncogene. The detection of point mutations correlated with diseases is currently performed by digestion of PCR products (PCR/RFLP) by using restriction endonucleases. It has been described here a method based modified on primers during the PCR, and using some restriction endonucleases (AatI, BanI, BanII, DraI, DraIII, EaeI, PstI and SacII) which create a restriction fragment length polymorphism (RFLP) indicative of the studied mutation. The present study used the electromagnetic fields (4.5 Hz); PCR/RFLPs technique was selected as a biomarker to evaluate the effect of exposure to electromagnetic fields in implanted Ehrlich tumor of female BALB/C mice. Eighty mice were used and divided into four groups (20 each); normal, exposed (exposed to 4.5 Hz), infected (normal infected by Ehrlich tumor) and infected exposed (infected exposed to 4.5 Hz). DNA genome was extracted and ETS-1 oncogene detected (~4460 bp). AatI, BanII and EaeI restriction endonucleases did not differentiate between the PCR products (ETS-1 genes) of the four groups (normal, exposed, infected and infected exposed mice groups). DraIII, SacII, PstI, BanI and DraI differentiated between the four groups. The results proved that the electromagnetic fields could treat the tumor and PCR\RFLPs were able to be a useful diagnostic technique.

DOI

10.21608/ejhm.2006.18040

Keywords

Electromagnetic Field, Cancer, ETS-1 oncogene, PCR/RFLP

Authors

First Name

Amany A.

Last Name

Tohamy

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Affiliation

Department of Zoology, Faculty of Science, Helwan University, Egypt.

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First Name

Mohammed H.

Last Name

Awwad

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Affiliation

Department of Zoology, Faculty of Science, Benha University, Egypt.

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First Name

Nahed M.

Last Name

El-Abiad

MiddleName

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Affiliation

Biological Application Department, Nuclear Research center, Atomic Energy Authority, Cairo, Egypt.

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First Name

Abdel-Monsef A.

Last Name

Elhadary

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-

Affiliation

Biological Application Department, Nuclear Research center, Atomic Energy Authority, Cairo, Egypt.

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Volume

22

Article Issue

1

Related Issue

3739

Issue Date

2006-01-01

Receive Date

2018-10-31

Publish Date

2006-01-01

Page Start

155

Page End

168

Print ISSN

1687-2002

Online ISSN

2090-7125

Article File

EJHM_Volume 22_Issue 1_Pages 155-168.pdf

PDF . 691.9kB

Link

https://ejhm.journals.ekb.eg/article_18040.html

Detail API

https://ejhm.journals.ekb.eg/service?article_code=18040

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Original Article

Type Code

606

Publication Type

Journal

Publication Title

The Egyptian Journal of Hospital Medicine

Publication Link

https://ejhm.journals.ekb.eg/

MainTitle

Molecular Biological Studies on the Effect of the Electromagnetic Fields on ETS-1 Oncogene

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Article

Created At

22 Jan 2023