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18033

Do Molecular Markers Predict the Electromagnetic Field Treatment of Cancer Through p53 Suppressor Gene?

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Last updated: 24 Dec 2024

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Abstract

In recent years there have been enormous studies made toward understanding diagnosis and treatment of cancer. Although there has been a great deal learned about cancer, the treatments available for it have not progressed nearly as much. Attempted removal of the tumor followed by chemotherapy and radiotherapy still prevail as the most effective treatments used. The present study used the electromagnetic fields (4.5 Hz) to treat tumor implanted in mice. The Polymerase Chain Reaction/Restriction Fragment Length Polymorphisms (PCR/RFLPs) technique was selected as a biomarker to evaluate the effect of exposure to electromagnetic fields in implanted Ehrlich tumor of female BALB/C mice. Eighty mice used and divided into four groups (20 each); control, radiated (control exposed to 4.5 Hz), infected (control infected by Ehrlich tumor) and infected exposed (infected exposed to 4.5 Hz). The duration of exposure was for two hours every two days. Electromagnetic field exposure includes group 2 and group 4. DNA genome was extracted and p53 suppressor gene detected (~2130 bp). AatI, BanII, EaeI restriction endonucleases did not differentiate between the PCR products (p53 genes) of the four groups (control, radiated, infected and infected exposed mice groups). BanI, DraI, DraIII, HaeII and PstI differentiated between the four groups. The results approved that the electromagnetic fields could treat the tumor and PCR\RFLPs could be useful diagnostic technique.  

DOI

10.21608/ejhm.2006.18033

Keywords

Electromagnetic Field, Cancer, p53 gene, PCR/RFLP

Authors

First Name

Mohammed H.

Last Name

Awwad

MiddleName

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Affiliation

Department of Zoology, Faculty of Science, Benha University, Egypt

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First Name

Amany A.

Last Name

Tohamy

MiddleName

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Affiliation

Department of Zoology, Faculty of Science, Helwan University, Egypt.

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Orcid

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First Name

Nahed M.

Last Name

El-Abiad

MiddleName

-

Affiliation

Biological Application Department, Nuclear Research center, Atomic Energy Authority, Cairo, Egypt.

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First Name

Abdel-Monsef A.

Last Name

Elhadary

MiddleName

-

Affiliation

Biological Application Department, Nuclear Research center, Atomic Energy Authority, Cairo, Egypt.

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Volume

22

Article Issue

1

Related Issue

3739

Issue Date

2006-01-01

Receive Date

2018-10-31

Publish Date

2006-01-01

Page Start

111

Page End

125

Print ISSN

1687-2002

Online ISSN

2090-7125

Article File

EJHM_Volume 22_Issue 1_Pages 111-125.pdf

PDF . 455.8kB

Link

https://ejhm.journals.ekb.eg/article_18033.html

Detail API

https://ejhm.journals.ekb.eg/service?article_code=18033

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Original Article

Type Code

606

Publication Type

Journal

Publication Title

The Egyptian Journal of Hospital Medicine

Publication Link

https://ejhm.journals.ekb.eg/

MainTitle

Do Molecular Markers Predict the Electromagnetic Field Treatment of Cancer Through p53 Suppressor Gene?

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Article

Created At

22 Jan 2023