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Macrophage Migration Inhibitory Factor in Systemic Lupus Erythematosus and its Expression in Lupus Nephritis

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Last updated: 24 Dec 2024

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Abstract

Objective: the aim of this study was to access the potential involvement of MIF in SLE, its relationship with corticosteroid dose, also, to measure serum and urinary MIF levels in SLE as well as detecting renal MIF expression in SLE GN. Methods: Serum and urine MIF concentrations were measured by enzyme-linked immunosorbent assay in 20 SLE female patients with lupus nephritis , World Health Organization class II, III, IV, with mean age of 35.1510.42 years and in 10 normal healthy, age matched, female volunteers. All patients were subjected to detailed clinical assessment and laboratory investigations. Serum and urinary MIF concentrations were measured by ELISA technique. Renal MIF expression was assessed by immunostaining of biopsy tissue. Univariate and multivariate regression analysis were used to examine the associations between serum and urine MIF concentrations, renal MIF expression, disease-related indices of SLE and corticosteroid use. Results: A statistically significant 2.98-fold-increase was detected in mean urinary MIF (U MIF) levels in SLE patients compared to controls. While, mean Serum MIF (S MIF) showed no significant difference between cases & control. Both S & U MIF concentrations were positively correlated with SLICC/ACR DI but not with SLEIDAI. Corticosteroid doses showed a highly positive correlation with S MIF, serum creatinine & SLICC/ACR DI. Also a positive correlation was found between the different histopathologic grades of renal affection & the U MIF. Immunohistochemistry staining of all normal kidney specimens showed that MIF is constitutively weakly expressed by some glomerular & parietal epithelial cells & by most tubular epithelial cells. In contrast, there was a significant increase in glomerular & tubular MIF protein staining in SLE nephropathy. This increased MIF expression correlated positively with both S MIF and U MIF, SLICC/ACR DI & the daily steroid dose Conclusion: This study shows that serum MIF is over-expressed in SLE patients and that the urine MIF concentration is significantly increased in SLE World Health Organization class IV patients and correlates with the degree of renal injury. Thus, urine MIF levels reflect MIF expression within the kidney.  

DOI

10.21608/ejhm.2006.17911

Keywords

SLE, MIF, GN, Renal biopsy

Authors

First Name

Nahla

Last Name

Khattab

MiddleName

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Affiliation

Health Radiation Research Departments Faculty of Medicine Ain Shams University

Email

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City

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Orcid

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First Name

Nihad A.

Last Name

El Nashar

MiddleName

-

Affiliation

Health Radiation Research Departments Faculty of Medicine Ain Shams University

Email

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City

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Orcid

-

First Name

Nevine

Last Name

Badr

MiddleName

-

Affiliation

National Centre for Radiation Research & Technology; Physical Medicine & Rehabilitation Department

Email

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City

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Orcid

-

First Name

Hanan

Last Name

Fahmy

MiddleName

-

Affiliation

National Centre for Radiation Research & Technology; Physical Medicine & Rehabilitation Department

Email

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City

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Orcid

-

First Name

Ragia

Last Name

Fahmy

MiddleName

-

Affiliation

Ain Shams University; Early Cancer d Dtection Unit Department, Ain Shams University Hospitals.

Email

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City

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Orcid

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First Name

Amal

Last Name

Alouba

MiddleName

-

Affiliation

Early Cancer d Dtection Unit Department, Ain Shams University Hospitals.

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Orcid

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Volume

24

Article Issue

1

Related Issue

3737

Issue Date

2006-07-01

Receive Date

2018-10-30

Publish Date

2006-07-01

Page Start

484

Page End

500

Print ISSN

1687-2002

Online ISSN

2090-7125

Article File

EJHM_Volume 24_Issue 1_Pages 484-500.pdf

PDF . 482.7kB

Link

https://ejhm.journals.ekb.eg/article_17911.html

Detail API

https://ejhm.journals.ekb.eg/service?article_code=17911

Order

7

Type

Original Article

Type Code

606

Publication Type

Journal

Publication Title

The Egyptian Journal of Hospital Medicine

Publication Link

https://ejhm.journals.ekb.eg/

MainTitle

Macrophage Migration Inhibitory Factor in Systemic Lupus Erythematosus and its Expression in Lupus Nephritis

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Article

Created At

22 Jan 2023