This study was designed to assessment nutrition status and blood glutathione (GSH) level for preschool children. Subjects and methods: The study included 70 (boys and girls preschool children) at aged from 2-5 years. Children was randomly selected from the out patients clinic at the National Nutrition Institute Cairo. Weigh and height were measured for them to evaluate the effect of nutrients on bodies, dietary intake was collected for the children were subjected to estimation of (Energy, Protein, Fat, Carbohydrate, vitamins A, folic acid and minerals iron, zinc and selenium) in their daily diet. Blood samples were collected to determine hemoglobin, glutathione and total protein concentration. Results: The dietary analysis showed that, every nutrient was lower than the requirement except total protein was higher than the recommend. Stunting showed (25.5%) of boys and (20%) of girls, underweight (23%) of boys and 14% of girls were the problems among preschool children. A glutathione deficiency was found among 97% of boys and 100% of girls. The hemoglobin ratio 77.1% from children was equal or less than normal concentration. Total protein noticed 82.9% of boys and 85.7 of girls in normal value. Conclusion: There was little quantity of nutrients intake, glutathione level and growth. The study can be recommended to improve their daily dietary intake and nutrition habits by education programs for their parents or supplement of studied cases with special ferrous and protein specially contains sulphur amino acids in daily diet to cover Recommended Dietary Allowances and can improve tissue GSH concentration.