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The protective effect of L-tryptophan versus alpha lipoic acid against L-arginine-induced experimental acute pancreatitis in albino rats

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Last updated: 24 Dec 2024

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Abstract

Aim of the study: This study was conducted to investigate the possible protective effects of L- treptophan "a precursor of melatonin" and alpha lipoic acid against L- arginine-induced experimental acute pancreatitis in albino rats. Methods: Fourty adult male albino rats (200- 250g) were randomized into 4 groups (n= 10). Group I, the control group was given 0.9% saline intraperitoneally (i.p). Group II, was given 500 mg/100g L-arginine (i.p) as a single dose to induce acute pancreatitis. Group III: was given 250mg/kg L-tryptophan (i.p) 30 min prior to L- arginine injection. Group IV: was given 50mg/kg alpha lipioc acid(i.p) 30 min prior to L-arginine. Before scarifice, blood samples were obtained from all groups to assay serum amylase and interleukin 6. Animals were sacrificed after 6 hours. For the histopathological study, pancreatic tissue was prepared for histological (H&E, PAS) histochemical ( Tween stain for lipases) and immunohistochemical ( Bax stain for apoptosis) techniques. Both qualitative and quantitative analyses were done to assess the degree of acinar cells affection Results: It was revealed that serum amylase and interleukin 6 in group II rose rapidly. Microscopically, severe acinar cells degeneration, interstitial edema, diffuse bleeding and inflammatory infiltration were demonstrated. These changes were markedly improved with the administration of both L- tryptophan and alpha lipoic acid. Conclusion: It was concluded that both L- tryptophan & alpha lipoic acid reduced the effects of L-arginine-induced acute pancreatitis with better protection achieved by L-tryptophan administration.

DOI

10.21608/ejhm.2007.17778

Authors

First Name

Lamia M.

Last Name

Farghaly

MiddleName

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Affiliation

Departments of Histology Faculty of Medicine, Suez Canal University

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Orcid

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First Name

Nagwan A.

Last Name

Sabak

MiddleName

-

Affiliation

Biochemistry Faculty of Medicine, Suez Canal University.

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Orcid

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First Name

Naglaa A.

Last Name

El-sherbeny

MiddleName

-

Affiliation

Pharmacology, Faculty of Medicine, Suez Canal University.

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Volume

26

Article Issue

1

Related Issue

3715

Issue Date

2007-01-01

Receive Date

2018-10-28

Publish Date

2007-01-01

Page Start

31

Page End

45

Print ISSN

1687-2002

Online ISSN

2090-7125

Article File

EJHM_Volume 26_Issue 1_Pages 31-45.pdf

PDF . 1.3MB

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https://ejhm.journals.ekb.eg/article_17778.html

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https://ejhm.journals.ekb.eg/service?article_code=17778

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Original Article

Type Code

606

Publication Type

Journal

Publication Title

The Egyptian Journal of Hospital Medicine

Publication Link

https://ejhm.journals.ekb.eg/

MainTitle

The protective effect of L-tryptophan versus alpha lipoic acid against L-arginine-induced experimental acute pancreatitis in albino rats

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Article

Created At

22 Jan 2023