Beta
ManageSearchChatDiscover
17719

Aspergillus fumigatus and Cylindrocarpon candidum fungi induced apoptosis in HepG 2 cell line through activation of caspases enzymes

Article

Last updated: 24 Dec 2024

OverviewSimilar Items

Subjects

-

Tags

-

Abstract

Introduction: Many investigations are now interested to discover naturally occurring compounds, which can be used for the prevention and treatment of cancer. Most natural products which may be used as adjuvant therapy or to reduce the side effect of chemotherapy and radiotherapy. More than 300 products obtained from microorganisms have antitumor activities. Results: In the study we isolated N-(3-4-Dichlorophenyl) 2-Methyl, 2,3Dihydroxypropio amide from Aspergillus fumigatus and 2.4.6. Triphenyl pyridine from Cylindrocarpon candidum and investigate the cytotoxic effect and apoptotic effect on HepG2 cell line. The results revealed high cytotoxic effect at the concentration of 400μg/ml for both N-(3-4- Dichlorophenyl) 2-Methyl, 2,3Dihydroxypropio amide and 2.4.6. Triphenyl pyridine and effect is increase with time of incubation. The apoptotic effect of both products were investigated by measurement the caspase enzymes, the results showed highest activity of caspase 3 and caspase 9. Also at concentration 400μg/ml in both products. Conclusion: From this data we observe that two isolated product have antitumor effect and this effect is related to the concentration of the products and incubation period. Also, the two products induce apoptosis through increase activation of caspase 3 and caspase 9 which lead to programmed of cell death. This study need to furthermore study on experimental animal to confirm our results.

DOI

10.21608/ejhm.2007.17719

Authors

First Name

Osman,

Last Name

M.E

MiddleName

-

Affiliation

Department of Botany and Microbiology, Faculty of science, Helwan University.

Email

-

City

-

Orcid

-

First Name

Khttab,

Last Name

O.H

MiddleName

-

Affiliation

Department of Botany and Microbiology, Faculty of science, Helwan University.

Email

-

City

-

Orcid

-

First Name

Abo EL-Nasser,

Last Name

A.A

MiddleName

-

Affiliation

Department of Botany and Microbiology, Faculty of science, Helwan University.

Email

-

City

-

Orcid

-

First Name

L,A,R,

Last Name

Sallam

MiddleName

-

Affiliation

National Research Center, Department of Microbiology and Natural Products Chemistry

Email

-

City

-

Orcid

-

First Name

Wafaa Abdallah

Last Name

Ahmed

MiddleName

-

Affiliation

Department of Cancer Biology, Cancer Institute, Cairo University.

Email

-

City

-

Orcid

-

Volume

27

Article Issue

1

Related Issue

3714

Issue Date

2007-04-01

Receive Date

2018-10-27

Publish Date

2007-04-01

Page Start

163

Page End

175

Print ISSN

1687-2002

Online ISSN

2090-7125

Article File

EJHM_Volume 27_Issue 1_Pages 163-175.pdf

PDF . 1.6MB

Link

https://ejhm.journals.ekb.eg/article_17719.html

Detail API

https://ejhm.journals.ekb.eg/service?article_code=17719

Order

4

Type

Original Article

Type Code

606

Publication Type

Journal

Publication Title

The Egyptian Journal of Hospital Medicine

Publication Link

https://ejhm.journals.ekb.eg/

MainTitle

Aspergillus fumigatus and Cylindrocarpon candidum fungi induced apoptosis in HepG 2 cell line through activation of caspases enzymes

Details

Type

Article

Created At

22 Jan 2023