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17662

Role of melatonin in modulation of oxidative stress induced by delta- aminolevulinic acid in adult male albino rats.

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Last updated: 24 Dec 2024

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Abstract

Backgrounds: Delta-aminolevulinic acid (ALA) is a heme precursor that accumulates in acute intermittent porphyria (AIP) due to enzymatic deficiencies in the heme biosynthetic pathway, its accumulation has been associated with several symptoms because it works as an endogenous source of reactive oxygen species, which can exert oxidative damage to cell structures. The present work was designed to examine the ability of melatonin, a well known antioxidant and a free radical scavenger secreted from the pineal gland, to revert ALA-promoted damage in brain, liver and kidney of rats. Results: The present data demonstrated that chronically ALA-treated rats (40 mg / kg body wt day after day for 14 days) exhibited very highly significant increases in malondialdehyde (MDA) and protein carbonyl (PC) whereas the level of glutathione (GSH) was significantly diminished in the tissue homogenates of all tested organs (brain, kidney & liver). Among antioxidant enzymes, superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GSH-Rd), glutathione-S-transferase (GST) activities were significantly diminished by ALA treatment. Intraperitoneal injection of melatonin (10 mg / kg body weight, every day for 14 consecutive days) significantly ameliorated all the tested parameters. Melatonin moderately increased SOD, CAT, GSH-Rd and GST activities, thereby counteracting the oxidative stress induced by ALA. Nevertheless, exogenous ALA caused a strong net rise in MDA and PC and a significant decrease in GSH when given together with ALA, melatonin antagonized these effects and largely protected the integrity of tissue structures. From the present data, the protection of melatonin against ALA oxidative stress is obviously, so it is well recommended to use melatonin in patients suffering from symptoms related to ALA accumulation.  

DOI

10.21608/ejhm.2007.17662

Keywords

Delta-aminolevulinic acid, Oxidative Stress, Melatonin, Malondialdehyde, protein carbonyl, glutathione, superoxide dismutase, catalase, glutathione-reductase, glutathione-S-transferase

Authors

First Name

El-Sawi,

Last Name

MR

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Affiliation

Zoology Department, Faculty of Science, Mansoura University.

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Orcid

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First Name

Badawy,

Last Name

ME

MiddleName

-

Affiliation

Zoology Department, Faculty of Science, Mansoura University

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Orcid

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First Name

El-Gharieb,

Last Name

NM.

MiddleName

-

Affiliation

Zoology Department, Faculty of Science, Mansoura University

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Volume

28

Article Issue

1

Related Issue

3713

Issue Date

2007-07-01

Receive Date

2018-10-26

Publish Date

2007-07-01

Page Start

233

Page End

272

Print ISSN

1687-2002

Online ISSN

2090-7125

Article File

EJHM_Volume 28_Issue 1_Pages 233-272.pdf

PDF . 346.5kB

Link

https://ejhm.journals.ekb.eg/article_17662.html

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https://ejhm.journals.ekb.eg/service?article_code=17662

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Original Article

Type Code

606

Publication Type

Journal

Publication Title

The Egyptian Journal of Hospital Medicine

Publication Link

https://ejhm.journals.ekb.eg/

MainTitle

Role of melatonin in modulation of oxidative stress induced by delta- aminolevulinic acid in adult male albino rats.

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Article

Created At

22 Jan 2023