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17703

Effects Of Aging On Pancreatic Islet Cell Function : An Experimental Immunohistochemical And Ultrastructural Study

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Last updated: 03 Jan 2025

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Abstract

A strong relationship between aging and diabetes mellitus has been clinically suggested, however, none of the previous published data had clearly focused on the age-related cytomorphological changes in the pancreas which are the goal of this study.Three groups of male apparently healthy rabbits have been used, ten animals each; classified as group-1 (3- 5months old); group-2 (9-12 months old) and group-3 (24-36 months old). After sacrification, sections from the pancreas were stained by Haematoxylin and Eosin (H&E), Gomori trichromic stain & ultrastructurally to detect aging histologic changes as well as immunohistochemically to identify insulin and glucagon secreting cells using their appropriate monoclonal antibodies . A progressive histological distortion with fibrosis and fatty changes were directly proportional to age, being mild in group-2 and severe in group-3. Morphometric studies by computerized image analysis showed that the mean number of islets was significantly higher in group2 (8.98±1.51), lowest in group-1 (5.08±1.48) and intermediate in group-3 (6.37±1.37). The mean diameter and square area of islets were significantly higher in group-2 compared to other groups (P< 0.05). The mean number of cells per islet & their secretary granules were significantly (P <0.05) higher in group-2, intermediate in group-1 and lowest in group-3.In contrast, the mean number of cells per islet and their secretory granules were insignificantly (P< 0.05) higher in group -2, intermediate in group-3 and lowest in group-1.Also, the /ratio (cells/cells) was greatest in group-2 (3.059:1), intermediate in group-1 (3.37:1), and lowest in group-3 (2.479:1). The increased number of cells may be due to a compensatory process to correct the hormonal feedback mechanism of insulin .The results of this work suggest that cells are generally more vulnerable to aging, an observation which might be correlated clinically with higher incidence of diabetes in older ages.

DOI

10.21608/ejhm.2007.17703

Authors

First Name

Ahmed H.

Last Name

Abdel-Rahman

MiddleName

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Affiliation

Departments of Pathology Al-Azhar Faculty of Medicine (Assuit& Cairo)

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First Name

Hassan Abdel-Hady

Last Name

Hassan

MiddleName

-

Affiliation

Departments of Pathology Al-Azhar Faculty of Medicine (Assuit& Cairo)

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City

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Orcid

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First Name

Mohamed A.

Last Name

Kasem

MiddleName

-

Affiliation

Histology Al-Azhar Faculty of Medicine (Assuit& Cairo)

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Orcid

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First Name

Hosam El-Din H.

Last Name

Osman

MiddleName

-

Affiliation

Anatomy Al-Azhar Faculty of Medicine (Assuit& Cairo)

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Orcid

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First Name

Hanan S.

Last Name

Abdel-Hamid

MiddleName

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Affiliation

Departments of Pathology Kasr El-Aini Faculty of Medicine (Cairo)

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Orcid

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First Name

Mohamed

Last Name

El- Basil

MiddleName

-

Affiliation

Internal Medicine Kasr El-Aini Faculty of Medicine (Cairo)

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Orcid

-

First Name

Mohammed A.

Last Name

Mashahit

MiddleName

-

Affiliation

Department of Internal Medicine Al-Fayoum University.

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Volume

29

Article Issue

1

Related Issue

3712

Issue Date

2007-10-01

Receive Date

2018-10-27

Publish Date

2007-10-01

Page Start

591

Page End

603

Print ISSN

1687-2002

Online ISSN

2090-7125

Article File

EJHM_Volume 29_Issue 1_Pages 591-603.pdf

PDF . 1.3MB

Link

https://ejhm.journals.ekb.eg/article_17703.html

Detail API

https://ejhm.journals.ekb.eg/service?article_code=17703

Order

11

Type

Original Article

Type Code

606

Publication Type

Journal

Publication Title

The Egyptian Journal of Hospital Medicine

Publication Link

https://ejhm.journals.ekb.eg/

MainTitle

Effects Of Aging On Pancreatic Islet Cell Function : An Experimental Immunohistochemical And Ultrastructural Study

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Article

Created At

22 Jan 2023