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17655

Biochemical, Histological And Histochemical Studies On The Effect Of Sodium Barbital On The Renal Tissue Of Albino Mice

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Last updated: 24 Dec 2024

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Abstract

Introduction:Anaesthetic drugs are nowadays used on a large scale in surgical operations as well as in other various medical purposes. Sodium barbital is a derivative of barbituric acid and is widely used on short surgical operations and other various medication. However, such anaesthetic drug has been reported to evoke many serious alterations as a result of its application. Materials and Methods:The experimental animals (30 mice-weighing 25-30 g) were divided into 3 groups (10/group), the first group served as a control group (i.e. injection with saline), while the other two groups were treated daily with the therapeutic dose of 60 mg/kg.b.wt sodium barbital (i.p.) for 7 days (short-term group) and 21 days (long-term group) as repeated daily doses. Blood sera and kidney samples were collected for physiological, histological and histochemical studies. Results:The results obtained showed a significant increase in urea, blood urea nitrogen, uric acid and creatinine levels in all treated groups. On the other hand serum total protein and albumin levels showed a significant decrease in both treated groups, while the globulin showed a significant decrease only in the long term group. The applied dose of sodium barbital caused histophathological alterations in the renal tissue mainly in the cortex such as damage and shrinkage of the Malpighian corpuscles, cloudy swelling and necrosis of the cells of the proximal convoluted tubules. Also, distal convoluted tubules exhibited degenerated features. In the histochemical studies, polysaccharides were progressively reduced in both short and long- term groups, while the total proteins showed a reduction in the short term group and considerably increase in the long term group. Conclusion: So these results came to conclusion that barbiturates should be prohibited and carefully used specially when prescribed as tranquilizer.  

DOI

10.21608/ejhm.2008.17655

Keywords

Barbital sodium, Kidney function, histopathology, histochemical (protein- polysaccharides.- Albino mice

Authors

First Name

Samia M.

Last Name

Sakr

MiddleName

-

Affiliation

Department of Biological Sciences, Faculty of Education, Ain Shams University

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Orcid

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First Name

Shadia Ali

Last Name

Radwan

MiddleName

-

Affiliation

Department of Biological Sciences, Faculty of Education, Ain Shams University

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Orcid

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First Name

Aziza M.

Last Name

El Wessemy

MiddleName

-

Affiliation

Department of Biological Sciences, Faculty of Education, Ain Shams University

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Orcid

-

First Name

Samira E

Last Name

El-Harras

MiddleName

-

Affiliation

High Institution of Applied Arts, 6th October City, Cairo, Egypt.

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Volume

30

Article Issue

1

Related Issue

3703

Issue Date

2008-01-01

Receive Date

2018-10-26

Publish Date

2008-01-01

Page Start

78

Page End

89

Print ISSN

1687-2002

Online ISSN

2090-7125

Article File

EJHM_Volume 30_Issue 1_Pages 78-89.pdf

PDF . 1.4MB

Link

https://ejhm.journals.ekb.eg/article_17655.html

Detail API

https://ejhm.journals.ekb.eg/service?article_code=17655

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8

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Original Article

Type Code

606

Publication Type

Journal

Publication Title

The Egyptian Journal of Hospital Medicine

Publication Link

https://ejhm.journals.ekb.eg/

MainTitle

Biochemical, Histological And Histochemical Studies On The Effect Of Sodium Barbital On The Renal Tissue Of Albino Mice

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Article

Created At

22 Jan 2023